kolkata ranges of dna concentration in plant

Complete Genes May Pass from Food to Human Blood

Jul 30, 2013· The plant DNA concentration shows a surprisingly precise log-normal distribution in the plasma samples while non-plasma (cord blood) control sample was found to be free of plant DNA. Citation: Spisák S, Solymosi N, Ittzés P, Bodor A, Kondor D, Vattay G, et al. (2013) Complete Genes May Pass from Food to Human Blood.

Addgene: DNA Quantification

If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample.

Banasri HAZRA | M.Sc., Ph.D. | Research Scientist 'C ...

Here, several derivatives of 1 were evaluated by the MTT assay and it was observed that the ethanolamine analogue (10) exhibited maximum cytotoxicity [50% inhibitory concentration (IC(50))=2.9 ...

Anirban CHAKRABORTY | University of Calcutta, Kolkata ...

Cytotoxicity, genotoxicity, and biochemical effects were evaluated in the plants Allium cepa, Nicotiana tabacum, and Vicia faba following exposure to ZnO nanoparticles (np; diameter, ~85 nm).

A high throughput DNA extraction method ... - Plant Methods

Jul 28, 2012· To precipitate the CTAB-DNA complex, the aqueous phase DNA extracts was diluted with 2 volumes of dilution buffer, bringing the NaCl concentration from 1.2 M (extraction buffer) down to about 0.4 M. This modification had consistently allowed the precipitation of copious amount of CTAB-DNA complex in a range of plant samples that vary in tissue ...

Publication International - CSIR-NEERI Kolkata Zonal ...

Information About Search Information About Search1. If you want to search any staff of NEERI then you can directly search with Staff Name e.g. Pankaj Sharma 2. Please Remove quotes around phrases to search for each word individually.

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LABORATORY 3. PREPARATION OF PLASMID DNA Exercise 1 ...

determine the concentration of DNA in solution by applying the Beer‐Lambert law. However, the Beer‐Lambert equation is only linear for absorbances between 0.1 and 1.0. This translates to concentrations between 10.0 ng/uL and 3700 ng/uL when using the Nanodrop ND‐1000. Samples outside of this range should be dried‐down or diluted to

DNA Purification | DNA Extraction Methods | Promega

Plant Genomic DNA Isolation . The Wizard® Magnetic 96 DNA Plant System (.# FF3760, FF3761) is designed for manual or automated 96-well purification of DNA from plant leaf and seed tissue. The Wizard® Magnetic 96 DNA Plant System has been validated with corn and tomato leaf as well as with canola and sunflower seeds.

Methods to maximise recovery of environmental DNA from ...

Jun 12, 2017· The environmental DNA (eDNA) method is a detection technique that is rapidly gaining credibility as a sensitive tool useful in the surveillance and monitoring of invasive and threatened species. Because eDNA analysis often deals with small quantities of short and degraded DNA fragments, methods that maximize eDNA recovery are required to increase detectability.

(PDF) ISOLATION AND CHARACTERIZATION OF POTENTIAL ...

isolation and characterization of potential phosphate solubilizing bacteria from soil samples of agricultural fields of dhapa, kolkata, west bengal. Experiment Findings (PDF Available) · March ...

Mobile antibiotic resistome in wastewater treatment plants ...

Mar 21, 2019· Wastewater treatment plants (WWTPs) are recognized as hotspots for horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs). Despite our understanding of the composition and distribution of ARGs in WWTPs, the genetic location, host, and fate of ARGs remain largely unknown. In this study, we combined Oxford Nanopore and Illumina metagenomics sequencing to …

What is the role of salt, isopropanol and ethanol in DNA ...

With ethanol, the DNA needs to be at a higher concentration to flocculate but the salt tends to stay soluble, even at cold temperatures. DNA falls out of solution in 35% isopropanol and 0.5M salt.

Enhanced transcription rates in membrane-free protocells ...

Jul 16, 2013· However, in coacervates (where the PEG concentration exceeds 12–15 wt%), transcription is fully active: Fig. 3B shows the increase in fluorescence intensity for dilute lysate and coacervates as a function of time for a DNA concentration of 160 pM in the starting droplet (see Fig. S6 for mRNA production from 40, 60, 80, 120, and 160 pM DNA).

Cell - Transport across the membrane | Britannica

Cell - Cell - Transport across the membrane: The chemical structure of the cell membrane makes it remarkably flexible, the ideal boundary for rapidly growing and dividing cells. Yet the membrane is also a formidable barrier, allowing some dissolved substances, or solutes, to pass while blocking others. Lipid-soluble molecules and some small molecules can permeate the membrane, but the lipid ...

Fermentation Technology - Bioprocessing

Enzymes have been produced commercially from plant, animal and microbial sources. Microbial enzymes have the enormous advantage of being able to produced in large quantities by established fermentation techniques. The advent of recombinant DNA technology has enabled enzymes of animal origin to be synthesized by m.o. e.g insulin.

Improving Photosynthesis | Plant Physiology

Photosynthesis is the basis of plant growth, and improving photosynthesis can contribute toward greater food security in the coming decades as world population increases. Multiple targets have been identified that could be manipulated to increase crop photosynthesis. The most important target is Rubisco because it catalyses both carboxylation and oxygenation reactions and the majority of ...

A simple and efficient genomic DNA extraction protocol for ...

Mar 01, 2015· 1. Introduction. Plants produce secondary metabolites that interfere not only with extraction of high quality genomic DNA but also with the subsequent reactions such as PCR and related genetic analyses (Kotchoni and Gachomo, 2009, Kotchoni et al., 2011).The widely used genomic DNA extraction procedures rely on lengthy protocols that use hazardous chemicals or expensive …

ROLE OF IRON IN PLANT GROWTH AND METABOLISM

In plants, iron is involved in the synthesis of chlorophyll, and it is essential for the maintenance of chloroplast structure and function. There are seven transgenic approaches and combinations, which can be used to increase the concentration of iron in rice seeds.

PCR Troubleshooting Guide | Thermo Fisher Scientific - US

Choose DNA polymerases with high processivity, which display high tolerance to common PCR inhibitors carried over from soil, blood, plant tissues, etc. Insufficient quantity: Examine the quantity of input DNA and increase the amount if necessary. Choose DNA polymerases with high sensitivity for amplification.

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Nucleic acid quantitation - Wikipedia

In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to quantitate, or establish the concentration, of nucleic ...

Extracellular DNA Is Required for Root ... - Plant Physiology

Plant defense involves a complex array of biochemical interactions, many of which occur in the extracellular environment. The apical 1- to 2-mm root tip housing apical and root cap meristems is resistant to infection by most pathogens, so growth and gravity sensing often proceed normally even when other sites on the root are invaded. The mechanism of this resistance is unknown but appears to ...

Diverse and Widespread Contamination Evident in the ...

Oct 29, 2014· Finally, although the authors did find hundreds of reads matching plants in a maternal plasma sample, and none in a matched sample of whole fetal blood, the concentration of DNA in whole blood is tens-of-thousands-fold higher than that in blood plasma (approximately 60 µg/ml vs. 21ng/ml, ).

Jayaraman Vinayagam's research works | Indian Institute of ...

Jayaraman Vinayagam's 27 research works with 134 citations and 2,717 reads, including: Isobenzofuranone derivative JVPH3, an inhibitor of L. donovani topoisomerase II, disrupts mitochondrial ...

Mechanism of Salinity Tolerance in Plants: Physiological ...

Within the vacuole K + concentration ranges between 10 mM and 200 mM. The vacuole serves as the largest pool of K + within the plant cell. K + plays a major role in maintaining the turgor within the cell. It is transported into the plant cell against the concentration gradient via K …

High‐throughput isolation of circulating tumor DNA: a ...

Dec 22, 2018· Fig. S3. Performance of the MX platform using increasing DNA input (0, 15, and 60 ng·mL −1 fragmented cell line DNA has been spiked in HBD plasma). The effects on (A) cfDNA concentration (ng·mL −1 plasma) measured by TERT qPCR, (B) recovery efficiency measured by plant DNA qPCR, (C) total number of mutant molecules, and (D) VAF are shown. Boxes (interquartile ranges…

Absolute quantitation of microbiota abundance in ...

Jun 19, 2018· Effect of added spikes before and after DNA isolation on microbial quantitation. P synthetic spike DNA was added to samples prior to DNA isolation (a–c) in a concentration gradient (a–b) of 14 (circle), 34 (square), 70 (upward triangle), 140 (downward triangle) and 340 (diamond) pg per Rhizobium culture (1 ml) or soil (1 g) and (c) 39 (square) pg per soil (1 g) and then co-isolated with ...

DNA Extraction Protocol for Plants with High Levels of ...

Pure DNA typically shows a symmetrical peak at 260 nm and a smooth profile. Polysaccharides and other secondary metabolites, often copurified with plant DNA isolated using traditional methods, can interfere with OD readings (A 260 / A 280), leading to errors in determination of concentration and purity.

Nucleic acid purification from plants, animals and ...

Nov 21, 2017· Plant pathogen diagnostics: immunological and nucleic acid-based approaches. Ann Appl Biol. 2004;145: 1–16. View Article Google Scholar 2. Allen G, Flores-Vergara M, Krasynanski S, Kumar S, Thompson W. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide.

Total Genomic DNA | Norgen Biotek Corp.

Purified DNA is suitable for sensitive downstream applications including PCR, qPCR, genotyping and sequencing Norgen's Total genomic DNA Purification Kits allow for the isolation of total genomic DNA of all sizes from a wide range of sample inputs including cells, tissues, urine, blood, stool, FFPE samples, plants …

DNA Extraction & Purification/DNA Extraction from Plants ...

Plant Nuclear DNA Preparation (Lazo Lab, USDA) This method can be used to prepare Petunia cv. Rose du Ciel nuclear DNA suitable for restriction enzyme analysis and cloning. Chloroplast DNA contamination will vary depending on the genotype. Greater chloroplast DNA contamination results when this method is used with the "Mitchell" line of Petunia.

How to determine the concentration and purity of a DNA ...

Impurities in DNA may lead to inaccurate measurement of DNA concentration and could potentially inhibit subsequent labelling reactions. One of the common methods of measuring nucleic acid purity and concentration are spectrophotometric absorbance of a sample at 260nm compared to the value measured at 280nm .

Mutagenesis as a Tool in Plant Genetics, Functional ...

Typically, one seed from each line is sown to produce the M 2 population and, DNA is isolated from every single M 2 plant. Provided the number of mutations per genome is high enough and the size of the population is large enough, it is likely that a mutated allele of all genes in the genome exists somewhere in the population.